ABSTRACT. The present study aimed to evaluate the in vitro antimicrobial activity of pharmacologically important Adhatoda vasica plant extracts. Plant Adhatoda vasica L. Nees (Acanthaceae) commonly known as Malabar nut is an evergreen two – three m tall shrub, sometime used as. Adhatoda vasica (L.) (Acanthaceae) is used in the indigenous system of medicine in India. The alkaloid Vasicine was isolated from ethanolic extract of the .

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Journal of Natural Product and Plant Resources. Journal List Biomed Res Int v.

Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

The structure of the isolated compound Vasicine and Vasicine acetate Figure 1. The extract was filtered through Whatman No. Antimicrobial and antimycobacterial activities of methyl caffeate isolated from Solanum torvum Swartz. Extraction Shade dried and coarsely powdered leaves of A. The cytotoxicity was determined according to the method of Balachandran et al. Two mobile phases A and B were used at flow rate of 1. Accumulation of vasicine and vasicinone in tissue cultures of Adhatoda vasica and evaluation of the free radical-scavenging activities of the various crude extracts.

Essential oils of the leaves of A.

Zones of inhibition were recorded in millimetres and the experiment was repeated twice. Estimates of worldwide burden of cancer in Investigation on antifeedant and anthelmentic potential of Adhatoda vasica nees. Support Center Support Center.

The column was eluted with chloroform and chloroform: Acanthaceae is used in the indigenous system of medicine in India.


International Journal of Cancer. Minimum inhibitory concentration of Vasicine acetate against tested bacteria and fungi.

HPLC chromatograms of the ethanol extract of the leaves adhxtoda Adhatoda vasica. In the present communication we report the antimicrobial, antioxidant, and anticancer effects of Vasicine acetate acetylated from Vasicine obtained from A.

Cytotoxicity of each sample was expressed as IC 50 value. The leaves are used to treat malarial fever, chronic fever, intrinsic hemorrhage, cough, asthma, leprosy, skin diseases, and piles [ 6 ]. The chemicals are given in delta scale with TMS as an internal standard. Vasicine acetate showed minimum inhibitory concentration values against bacteria; they were M.

Cytotoxic effect of Vasicine acetate against A lung adenocarcinoma cancer cell line.

The free radical scavenging activity was calculated as follows:. Antifeedant and toxic activity of Adhatoda vasica leaf extract against Spodoptera littoralis Lep.

Conclusion Vasicine acetate was obtained by acetylation of Vasicine recovered from A. International Journal of Green Pharmacy. The physical and spectroscopic data were compared with those reported in the literature [ 2122 ]. BHT is used as standard of antioxidant. Cytotoxic studies against A lung adenocarcinoma cancer cell line showed Vasicine acetate to be the most potent compound Table 4. Antimicrobial Activity Antibacterial and antifungal activities were carried out using disc diffusion method [ 16 ].

DPPH 2,2-diphenylpicrylhydrazyl radical scavenging activity of Vasicine acetate was determined based on the method of Wang et al.

Antibacterial activity of adhatoda vasica leaf extract

The mobile phase was filtered through a 0. Effect of Adhatoda vasica massage in pyorrhoea. The combined chloroform extract was washed with water, dipped, and dried over anhydrous sodium sulphate.


The structure was elucidated using spectroscopic methods: The solutions were filtered through a membrane filter pore size 0. Streptomycin was used as positive control for bacteria and Ketoconazole as positive control for antimicrobiak.

The spot turned orange red on spraying with Dragendorff’s reagent. Structure of the compounds: Indian Journal of Natural Products and Resources. The required concentrations, Ignacimuthu123 C. Potent uterine activity of alkaloid vasicine. Ray of hope for cancer patients.

Antibacterial Activity of Leaf Extracts of Adhatoda vasica – MAHE Digital Repository

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Introduction Natural products still remain the most important source for discovery of new and potential drug molecules. Vasicine acetate exhibited good zone of inhibition against bacteria: Natural products still remain the most important source for discovery of new and potential drug molecules. A voucher specimen No.

BoxRiyadhSaudi Arabia. International Journal of Crude Drug Research.

Sankar2 and K. The MIC for fungi antimicdobial defined as the lowest extract concentration, showing no visible fungal growth after incubation time. Mobile phase A consisted of water with 0.