ANTIMICROBIAL ACTIVITY OF ADHATODA VASICA PDF

ANTIMICROBIAL ACTIVITY OF ADHATODA VASICA PDF

ABSTRACT. The present study aimed to evaluate the in vitro antimicrobial activity of pharmacologically important Adhatoda vasica plant extracts. Plant Adhatoda vasica L. Nees (Acanthaceae) commonly known as Malabar nut is an evergreen two – three m tall shrub, sometime used as. Adhatoda vasica (L.) (Acanthaceae) is used in the indigenous system of medicine in India. The alkaloid Vasicine was isolated from ethanolic extract of the .

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The physical and spectroscopic data were compared with those reported in the literature [ 15 ]. The plant was identified by Dr. BoxRiyadhSaudi Arabia.

Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Classical Uses of Medicinal Plants. Al-Dhabi1 C. Instruments All melting point values are uncorrected and taken by open capillary method anitmicrobial a heating block Instrument.

Abstract Adhatoda vasica L. Antimycobacterial activity of two natural alkaloids, vasicine acetate and 2-acetyl benzylamine, isolated from Indian shrub Adhatoda vasica Ness.

The combined chloroform extract was washed with water, dipped, and dried vasoca anhydrous sodium sulphate. Extraction Shade dried and coarsely powdered leaves of A. Cytotoxicity of each sample was expressed as IC 50 value. The spot turned orange red on spraying with Dragendorff’s reagent.

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Cytotoxic studies against A lung adenocarcinoma cancer cell line showed Vasicine acetate to be the most potent compound Table 4. Streptomycin was used as positive control for adhatpda and Ketoconazole as positive control for fungi.

Microbial Organisms The following Gram acyivity, Gram positive bacteria, clinical isolates, and fungi were used for the experiment: The cytotoxicity was determined according to the method of Balachandran et al.

DPPH 2,2-diphenylpicrylhydrazyl radical scavenging activity of Vasicine acetate was determined based on the method of Wang et al.

The antifungal agent Ketoconazole for fungi and antibacterial agent Streptomycin for bacteria were included in the assays as positive controls. Glossary of Indian Medicinal Plants. Vasicine acetate showed minimum inhibitory concentration values against bacteria: Preliminary phytochemical and antibacterial screening of crude extract of the leaf vasia Adhatoda vasica. HPLC chromatograms of the ethanol extract of the leaves of Adhatoda vasica.

Cytotoxic A and antimicrobial effects of Methylobacterium sp. Journal List Biomed Res Int v. The structure was elucidated using spectroscopic methods: Materials and Methods 2.

Results and Discussion The ethanol extract of A. The alkaloid Vasicine was isolated from ethanolic extract of the leaves of A. Investigation on antifeedant and anthelmentic potential of Adhatoda vasica nees. International Journal of Crude Drug Research. Indian Journal of Medical Research. Ignacimuthu12antimircobial C.

Vasicine acetate showed antimycobacterial activity [ 15 ]. Mobile phase A consisted of water with 0.

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The plant is used in the indigenous system of medicine in India and is a well-known expectorant in both Ayurvedic and Unani Systems of Medicine [ 45 ]. The MIC for fungi was defined as the lowest extract concentration, showing no visible fungal growth after incubation time. Medicinal plants are important sources of practical drugs for people throughout the year.

The extract was filtered through Whatman No. The required concentrations, Accumulation of vasicine and vasicinone in tissue cultures of Adhatoda vasica and evaluation of the free radical-scavenging activities of the various crude extracts.

Vasicine acetate was obtained by acetylation of Vasicine. Clinical and Laboratory Standards Institute; Ray of hope for cancer patients.

Antibacterial Activity of Leaf Extracts of Adhatoda vasica – MAHE Digital Repository

Published online Jan Open in a separate window. Other constituents include vitamin C, saponins, flavonoids as well as steroids, and fatty acids [ 13 ]. Minimum inhibitory concentration of Vasicine acetate against antimicrobiap bacteria and fungi. The chemicals are given in delta scale with TMS as an internal standard.