Genencor International (Danisco A/S) in the US was developing I V, In addition, there was negligible IgG antibody response to the variant interferon-β. Herceptin IgG human antibody were made and transformed into Trichoderma reesei. Genencor International, a Danisco Company. Page. GENENCOR INTERNATIONAL PALO ALTO CA Initial genetic constructs for the Herceptin IgG human antibody were made and transformed into Trichoderma.

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The pH was adjusted to 9.

Preferred sites include sites not in critical conformation determining regions and sites disposed away from the subsite s of the enzyme. However, rapid clearance could be caused by interactions with other receptors. In cases where a permanent silent mutation is necessary to introduce a restriction site one may want to avoid the introduction of a rarely ig codon.

A “dendron” is a species of dendrimer having branches emanating from a focal point which is or can be joined to a core, either directly or through a linking moiety to genebcor a dendrimer.

In this instance, only one form of the heavy chain was seen on reducing SDS-PAGE, and this band had the same mobility as the higher-mobility nonglycosylated band described geenncor results not shown. A catalytic antagonist of a target molecule, said antagonist comprising a targeting moiety that specifically binds to said target molecule said targeting moiety being attached to an enzyme that degrades said target molecule to reduce binding of the target molecule to its cognate ligand and to said targeting moiety thereby resulting in the release of said antagonist thereby allowing said antagonist to bind and degrade another target molecule.


The dendrimer targeting moieties before coupling to the enzyme would preferably include methanethiosulfonates with simple branching such as:. High-yield expression of the recombinant, atrazine-specific Fab fragment KB by the methylotrophic yeast Pichia pastoris.


NaNO 312 g; KCl, 0. The synthesis of the biotin-MTS was accomplished as outlined in Example 8.

Minha Park Genencor International, Inc. Only dendritic cells have the capacity to induce activation in resting peripheral T cells [ genecor3 ]. Storage diseases can be treated by supplementing the “missing” enzymatic activity. In preferred genehcor, the method increases the dosage therapeutic window of said drug.

Mutation of the gene in order to change its sequence to conform to the desired sequence is accomplished e. GA-H, glucoamylase- heavy-chain fusion protein; GA-L, glucoamylase- light-chain fusion protein; H, antibody heavy chain; L, antibody light chain.

Enhanced immunogenicity of a functional enzyme by T cell epitope modification

Human PBMCs from two different donors were used in the analysis. Various preservatives are well known genencoor include, for example, phenol and ascorbic acid.

The serum from parent- and variant-immunized guinea pigs cross-reacted at both the protein and the peptide level. Ten disposable eppendorf vials were filled as shown in Table Two T cell epitopes were found in 5 of the 10 guinea pigs immunized with B. AcOEt, gradient elution, 8: This preferably resulting in a reduction or loss of activity of the target and release of the chimeric molecule.

Fungal cells were removed from culture broth by filtration through a cellulose pad K; Seitz with FW12 diatomaceous earth Eagle Pitcher as a filter aid. In in vivo applications, the catalytic inhibitors of this invention because they offer greater activity at lower concentration, are expected to show longer effective serum half-life and lower toxicities than “traditional” chimeric molecules.


Into a polypropylene test tube was weighed about 25 – 30 mg of lyophilized SC.

Enhanced immunogenicity of a functional enzyme by T cell epitope modification

The solution was cooled to RT and stirred for a further 2 h, during which time a white solid precipitated from solution. These enzymes have been well characterized as to their activity and specificity by us and by others [ 2627 ]. Pharmaceutically acceptable carriers can contain a physiologically acceptable compound that acts, for example, to stabilize the composition or to increase or decrease the absorption of the agent.

It was explained above, that a wide variety of pharmaceuticals act as antagonists of receptors or receptor mediated activity. GMP P-selectin stored in Weibel. Unlike antibody fragments, full-length IgG1 molecules include an N-linked glycosylation site in the Fc portion of the heavy chain.


After 15 min, the reactions in 4 for SC-Biotin started to visibly change genrncor color to pale yellow. Antibodies also provide useful targets for the catalytic antagonists of this invention. LiBr 80 mg, 0. It has been suggested that terminal Galf may be antigenic 29 ; therefore, elimination of this residue from glycan by an appropriate choice of a strain or manipulation of a strain would be beneficial.