KLONOWANIE DNA PDF

KLONOWANIE DNA PDF

Blog. 18 December Prezi Awards The best presentations have arrived. 5 December Do this, not that: Keynote speech. 28 November Wady i Zalety Klonowania Idea 1. Idea 2. Rozmnażanie bezpłciowe – naturalne klonowanie. Klonowanie zachodzi przez: Klonowanie DNA. KLONOWANIE Klony DNA służą do: Co to jest klonowanie? Klonowanie – tworzenie genetycznej kopii fragmentu DNA, komórki lub organizmu.

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The plasmid, which has a single cut site The target gene fragment, which has a cut site near each end. The promoter “points” towards the right, meaning that it will drive transcription of the DNA sequence that lies to the right.

In other cases, bacteria may be used as protein factories. Instead, we must collect DNA from several colonies and see whether each one contain the right plasmid. Thus, all of the bacteria are placed on an antibiotic plate to select for ones that took up a plasmid.

KLONOWANIE by Julia Umaru on Prezi

The basic steps are:. This is the desired plasmid from the ligation. But when we talk about cloning and DNA cloning we’re talking about something a little bit simpler. Well let’s say that this is a strand of DNA right over here and I’m just drawing it as a long, but this is a double-stranded, and I’ll just write it down, this is double stranded.

Przegląd: Klonowanie DNA

In one technique called affinity chromatographya mixture of molecules extracted from the lysed bacteria is poured through a columnor a cylinder packed with beads. The antibody in the column is designed to bind to our protein of interest, and not to any other molecules in the mixture.

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In the final step, after all kloonwanie non-target proteins have been washed away, the target proteins are released from the antibodies in the column, and the pure protein is collected for use. And so the plasmid that we’re placing in might have complimentary base pairs over the overhangs, which will allow it easier, it will become easier for them to react with each other if they have these overhangs.

The bacteria are given a heat shock, which causes some of them to take up a plasmid.

It’s identical copies of a piece of DNA. Klonowanie i rekombinacja DNA.

Transformation and selection of bacteria are key steps in DNA cloning. Insulin capture from human serum. A chosen colony is grown up into a large culture. And plasmids tend to be circular DNA so we will paste it into a plasmid. And there’s a bunch of restriction enzymes, and I personally find it fascinating that we as a civilization have gotten to the point that we can find and identify these enzymes and we know at what points of DNA that they can cut.

It appears that the heat shock causes the formation of pores in the bacterial membrane, through which the DNA molecules can pass. In CK biology advanced concepts. And the technique that’s typically done is giving some type of a shock to the system that makes the bacteria take up the plasmids. Bacteria without a plasmid will die, while bacteria carrying a plasmid can live and reproduce. And this is amazing because obviously DNA, this isn’t stuff that we can, you know, manipulate with our hands the way that we would copy and paste things with tape.

DNA molecules built through cloning techniques are used for many purposes in molecular biology. And so now you would have, after you applied the restriction enzymes, you will have just that gene. This is not a useful plasmid. Campbell biology edycja 10, strony Let’s say we are trying to insert a gene into a plasmid so it can be expressed in bacteria. A restriction enzyme is a DNA-cutting enzyme that recognizes a specific target sequence and cuts DNA into two pieces at or near that site.

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Thus, the protein is trapped in the column while other molecules from the bacteria flow through. Transformation is a key step in DNA cloning. The bacteria can then be lysed split open to release the protein. So if you have a bacteria, you have a bacteria right over here, it has its existing DNA, so this is its existing genetic material right over there, let me label this.

Diagram of a plasmid. The bacteria that make colonies should all contain a plasmid which provides antibiotic resistance.

The plasmid is introduced into bacteria via process called transformationand bacteria carrying the plasmid are selected using antibiotics. They recognize klonkwanie sequences and then we can figure klonkwanie well which restriction enzyme should we use to cut out different pieces of DNA, but we have gotten to that point as a civilization.

So you would see things like this, which would be many, many, many cells of bacteria, there would be colonies of bacteria. Because of these possibilities, it’s important to collect plasmid DNA from each colony and check to see if it matches the plasmid we were trying to build.

What is the point of making many copies of a DNA sequence in a plasmid?