Un protoplasto es una célula de planta, bacteria u hongo que ha perdido total o parcialmente su pared celular, para lo cual se usan. Cultivo y fusion de protoplastos []. Loyola Vargas, V.M. Rosell, C.H. (ed.) Villalobos Arambula, V.M. (ed.) Access the full text: NOT AVAILABLE. Lookup the . El poder seleccionar eficientemente las plantas híbridas producidas a partir de fusión de protoplastos es fundamental. Se han logrado muchos avances en el.

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Plant Cell Rep Plant regeneration from mono- and dihaploid potato genotypes often results in ploidy changes, but little aneuploidy; on the other hand, regenerated plants from tetraploid genotypes produce a wide range of aneuploidy Kumar, Protoplasts of both types are mixed in an equal proportion attaining a density of 5 x 10 4 tusion 2 x 10 5 protoplasts per ml.

Non-transgenic applications of plant tissue culture in potato. If the problem continues, please let us know and we’ll try to help. Improvements in regeneration from protoplasts of potato and studies on chromosome stability.

Fusion de Protoplastos by Arely Gutierrez on Prezi

When colonies had developed, alginate beads were despolimeryzed using 20 mM sodium citrate solution in sorbitol 0,3 M, pH 7,4 and gently shaking. Solanum, protoplast fusion, polyethylene glycol, somatic hybrid, RAPD analysis. Chromosome analysis The numbers of chromosome of S. In conclusion, somatic fusions between the cultivated potato Solanum tuberosum and the wild species S. Interspecific hybridization between the cultivated potato Solanum tuberosum subspecies tuberosum L.


Protoplasts fusion from S.

Cultivo y fusion de protoplastos [1990]

Due to that, fused and unfused protoplasts were cultured together. Protoplast-fusion derived Solanum cybrids: Isolation of Protoplasts from Tissues of day-old Seedlings of Arabidopsis thaliana ….

Identification of somatic hybrids of dihaploid S. Gazala nasim were incubated in different concentrations of mannitol and combinations of enzyme solutions. Isolation of plant DNA from fresh tissue. Somaclonal variation in potato. Characterization of somatic hybrids of potato by use of RAPD markers and isozyme analysis.

A rapid calli proliferation was observed after dusion developed. Please check your Internet connection and reload this page. Somatic hybrids were confirmed morphologically under greenhouse conditions, when intermediate characteristics between parent lines, such as shoot coloration, leaf shape and pubescence were observed. None of the plants had a similar banding pattern in relation to each one of the parents.

Literature cited Austin S. Protoplasts density is usually determined by haemocytometer.

An unexpected error occurred. These samples were obtained from rooted plants maintained in a greenhouse.

Cultivo y fusion de protoplastos

It has been suggested that protoplasts are more prone to chromosome instability because they undergo a long period of greater stress during initial stages of cell division and dedifferentiation Pijnaker and Sree Ramulu ; Carrasco et al.

Somatic hybridization in the genus Solanum: Universidad Nacional Agraria La Molina.

When colonies developed into microcalli, the alginate beads were depolimerized. After that, protoplasts settled down. Plant Cell Tissue Organ Cult Mantenga los protoplastos en el hielo durante 30 minutos. These results showed that all hybrids were aneuploids Fig. Nineteen plants were regenerated from calli Fig. Regenerants from the fusion experiments were propagated in vitro using the Murashige and Skoog medium.


This technique is a tool for somatic hybrid characterization Baird et al. The mixture was incubated for thirty minutes and then protoplasts were collected by centrifugation at protoplasfos during five minutes. These plants were multiplied in glass culture tubes for fuskon analysis.

You will only be able to see the first 20 protoplasttos. Results and discussion Protoplasts fusion and plant regeneration Protoplasts fusion from S. Soja Glycine max L. Under these conditions, calli hybrids follow the developmental pathway of the morphogenic parents. Scribd is the worlds largest social reading and publishing site. Plant Cell Rep 3: Plant Cell Tissue Organ Cult. You must be signed in to post a comment.

A revised medium for rapid growth bioassays with prottoplastos tissue culture. RAPD and chromosomal analysis of the hybrids are also presented. Unable to load video. Introduction Potato, Solanum tuberosum L.