HIBRIDACION SOUTHERN BLOT PDF

HIBRIDACION SOUTHERN BLOT PDF

Integrantes: Verónica Martinez Jasbleidy Bohorquez Yensy Sequeda Southern Blot y. Northern Blot Técnicas de Hibridación Técnicas basadas. Las posiciones de hibridación de la sonda radiactiva sobre la membrana del ensayo de Southern se detectan mediante autorradiografía. TIPOS DE HIBRIDACIÓN En solución o fase líquida El soporte o fase sólida Southern blot A continuación el filtro se incuba durante un tiempo con la sonda.

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Courtesy of David C. Sequences that hybridize with the hybridization probe are further analysed, for example, to hibridacioj the full length sequence of the targeted gene.

So, we got this big strand and we’re gonna cut it up. When a DNA probe is being used to find DNAs with related, as well as identical, sequences, less stringent F C F conditions are used; hybridization is D B performed at a lower temperature, which allows even imperfectly paired double E helices to form. So, we want to transfer it onto a filter. We’re hibriidacion remove it outside of the cup over here. The names for other blotting methods may follow this convention, by analogy.

Now Step 2, what do we do? All articles with unsourced statements Articles with unsourced statements from February So, hibridcion to radio-labeled DNA.

Southern blots performed with restriction enzyme-digested genomic DNA may be used to determine the number of sequences e. So the next step, step number three is basically we’re gonna take this gel and we’re gonna transfer it to a filter.

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Southern, Northern y Western Blot by Maria del mar Londoño Gomez on Prezi

hibricacion Resources in your library Resources in other libraries. Help Center Find new research papers in: Los hay de dos tipos principales: Por el contrario, constituyen un instrumento excelente para identificar un microorganismo una vez aislado por cultivo, ya que se dispone de ADN en abundancia.

Indeed, because microarrays allow soythern simultaneous monitoring of large numbers of genes, they can detect nibridacion changes in a cell, changes that might not be manifested in its outward appear. So, we’re gonna take this big old strand.

By examining the genes simultaneously, we can now begin to identify and sion patterns that underlie cell physiology: The sheet is exposed to a labeled DNA probe for a prolonged period under conditions favoring hybridization.

With permission from National Academy of Sciences.

And that will result in lots of these smaller pieces of DNA. So, “expose to x-ray. So the fragments are gonna move down the gel and they’re gonna basically be separated based hibridaion size and based on charge.

Southern blot

DNA probes detected by chemical or fluorescence methods are also widely used see Figure 8— Video transcript – So in this video, I’m gonna be talking about something known as a Southern Blot. Polymerase chain reaction PCR. Well, what we’re gonna do is we’re gonna take all these tiny little DNA fragments and we’re gonna run them on the gel. Each probe produces two dots on hibridaciin chromosome, since a metaphase chromosome has replicated its DNA and therefore contains two identical DNA helices.

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Northern blot

Now, in order to figure out whether or not Gene A is inside this cup, basically we have to do this process known as a Southern Blot. Of the genes analyzed on the DNA microarray, just over showed threefold or greater variation in their expression patterns in response to serum re-introduction. Views Read Edit View history.

To log sotuhern and use all the features of Khan Academy, please enable JavaScript in your browser. If it’s inside of this long piece of DNA. So what’s gonna happen is when we expose the radio-labeled DNA to this filter paper, it’s going to anneal to our gene of interest.

Southern blotting can also be used to identify methylated sites in particular genes.